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Human Tuberculosis Caused by Mycobacterium bovis, Taiwan

Human Tuberculosis Caused by Mycobacterium bovis, Taiwan

To the Editor: Mycobacterium bovis is one of the causative agents of tuberculosis (TB) in humans and animals. Drinking unpasteurized milk, eating undercooked meat, and close contact with infected animals are the main sources of infection for humans. Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping database (SpolDB 4) and are categorized into 3 main sublineages corresponding to ST prototypes 482, 683, and 479 (1).

Although an M. bovis surveillance program for farm animals has been implemented by the Taiwan Council of Agriculture, no surveillance system exists for human TB cases caused by M. bovis. To monitor the epidemiology of M. bovis in domestic animals, a regular tuberculin skin test (TST) is compulsory for cattle and sheep and optional for deer in Taiwan (2). In 2005, screening of Mycobacterium spp. infections by TST was performed for 111,412 cattle and 73,396 caprint and ovine herds, of which 188 (0.17%) and 148 (0.2%), respectively, were positive (2). We used spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unitsvariable number tandem repeat (MIRU-VNTR) methods to investigate human TB caused by M. bovis in Taiwan.

During 2004–2005, a total of 3,321 mycobacteria isolates from individual patients were sent to the reference laboratory for strain typing. Of the 3,321 patients, 2,427 (73.1%) were male, 903 (27.2%) were from eastern Taiwan, and 513 (15.4%) were aboriginal persons. The mean age of the patients was 58.7 years: 224 (6.7%) were <25 years of age, 667 (20.1%) were 25–44 years of age, 906 (27.3%) were 45–64 years of age, and 1,524 (45.9%) were >65 years of age.

Isolates were screened by using a GenoType kit (3) and multiplex PCR (4). To differentiate between M. bovis and M. bovis BCG strains, presence or absence of region of difference 1 was analyzed (5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands) following the manufacturer’s instructions (6). Spoligotyping profiles were analyzed by using Bionumerics software, version 4.51 (Applied Maths, Kortijk, Belgium). The resolved spoligotype was designated by comparing it to SpolDB4 (1). The MIRU-VNTR assay was performed by using a modified, high-throughput, 15-loci MIRU typing system that we developed (7).

Of the 3,321 patient isolates, 3,306 (99.5%) were M. tuberculosis and only 15 (0.5%) were M. bovis. Mean age of the 15 M. bovis–infected patients was 62.2 years (Table). Twelve (80%) patients were male, and 3 (20%) were female. Of these 15 patients, 10 (66.7%) had newly diagnosed TB and 5 (33.3%) had been treated with anti-TB drugs. Most (11/15, 73%) of the patients were from eastern Taiwan, where the reporting rate for TB was highest among the 4 regions of Taiwan; 60% (9/15) were aboriginal persons. Of the 15 patients, 13 (86.7%) had pulmonary TB, 1 had both pulmonary and extrapulmonary TB, and 1 had extrapulmonary TB. Only 2 patients (cases 2 and 3) had known contact with farm animals. Univariate analysis showed that region (eastern region 1.2% vs. other region 0.2%; odds ratio [OR] 7.4, 95% confidence interval [CI] (2.4–23.4) and ethnicity (aboriginal 1.8% vs. nonaboriginal 0.2%; OR 8.3, 95% CI 3.0–23.5) were associated with M. bovis infection but not age and sex. The association between region and M. bovis disappeared after controlling for age and ethnicity. Aboriginal ethnicity was the only factor significantly associated with TB caused by M. bovis after controlling for age (adjusted OR 12.7, 95% CI 4.2–38.9).

Table

Patient no.Age at diagnosis, ySexType of tuberculosisEthnicityRegion
153MPulmonaryAboriginalNorthern
271MPulmonaryHanCentral
376MPulmonaryHanCentral
484MPulmonaryHanSouthern
571FPulmonaryAboriginalEastern
655MPulmonaryAboriginalEastern
751MPulmonaryAboriginalEastern
865FPulmonaryAboriginalEastern
951MPulmonaryHanEastern
1090MPulmonaryHanEastern
1134MPulmonaryAboriginalEastern
1281MPulmonaryHanEastern
1364FPulmonaryAboriginalEastern
1444MBladder/urinaryAboriginalEastern
1543MPulmonary/miliaryAboriginalEastern
Characteristics of 15 patients with tuberculosis caused by Mycobacterium bovis, Taiwan, 2004–2005

Spoligotyping profiles of the 15 M. bovis isolates were typical of M. bovis with the absence of spacers 3, 6, 9, 16, 21, and 39–43 (8). Although 15 cases were reported separately from different regions of Taiwan, only 1 spoligotyping profile was identical to spoligotype ST 684 of the bovis sublineage. In addition, we identified 2 similar MIRU-VNTR profiles: 523–23232–42533–22 (13/14, 92.9%, cases 1–9, 11–13, and 15) and 523–22232–42523–22 (1/14, 7.1%, case 14). We were unable to obtain sufficient DNA from 1 strain (case 10) for MIRU-VNTR typing.

Aboriginal persons in Taiwan were more likely to have TB caused by M. bovis and had a 5-fold higher reporting rate for TB (9) than nonaboriginal persons. Environmental and genetic factors may be associated with a higher reporting rate for TB among aboriginal populations (10), but the contribution of M. bovis infection needs to be investigated. Because only 1 major spoligotype and 2 similar MIRU patterns were found in this case series, spread of a predominant clone in Taiwan is likely. In addition, because of insufficient epidemiologic data, we were unable to determine the proportion of cases caused by reactivation of latent infection and those caused by recent transmission. In our study population, M. bovis infection in humans appeared to be predominately indigenous in Taiwan because no imported case was noted. We are now genotyping M. bovis strains isolated from farm animals to help elucidate the source of infection and transmission of M. bovis in Taiwan.